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| 36. Endersby NM, Hoffmann AA, White VL, Ritchie SA, Johnson PH, Weeks AR. 2011. Changes in the genetic structure of Aedes aegypti (Diptera: Culicidae) populations in Queensland, Australia, across two seasons: implications for potential mosquito releases. Journal of Medical Entomology 48:999-1007. doi: 10.1603/ME10264. [PDF] This paper describes how patterns of genetic isolation (reflecting movement) among populations of Aedes aegypti in North Queensland change over time and reinforces the notion that inland populations are likely to be isolated
35. Hoffmann AA, Montgomery BL, Popovici J, Iturbe-Ormaetxe I, Johnson PH, Muzzi F, Greenfield M, Durkan M, Leong YS, Dong Y, Cook H, Axford J, Callahan AG, Kenny N, Omodei C, McGraw EA, Ryan PA, Ritchie SA, Turelli M, O’Neill SL. 2011. Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission. Nature 476: 454–457. [PDF] [News and Views] This paper describes how the wMel Wolbachia infection successfully invaded two wild mosquito populations in communities in far north Queensland, Australia.
34. Walker T, Johnson PH, Moreira LA, Iturbe-Ormaetxe I, Frentiu F, McMeniman CJ, Leong YS, Dong Y, Axford A, Kriesner P, Lloyd AL, Ritchie SA, O’Neill SL Hoffmann AA. 2011. The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations. Nature 476: 450-453
[PDF] [News and Views] This paper describes the successful transinfection of A. aegypti with the avirulent wMel strain of Wolbachia and its abilty to spread into contained cage populations of mosquitoes.
33. Hussain M, Frentiu FD, Moreira LA, O’Neill SL, Asgari S. 2011. Wolbachia uses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vector Aedes aegypti. Proceedings of the National Academy of Sciences USA. Published online before print May 16, 2011, doi: 10.1073/pnas.1105469108. [PDF] This paper shows how Wolbachia alters the host microRNA profile of the Aedes aegypti mosquito and provides insight into the mechanisms of host manipulation used by this widespread endosymbiont.
32. Thomas P, Kenny N, Eyles D, Moreira L, O'Neill SL, Asgari S. (2011). Infection with the wMel and wMelPop strains of Wolbachia leads to higher levels of melanization in the hemolymph of Drosophila melanogaster, Drosophila simulans and Aedes aegypti. Developmental and Comparative Immunology 35: 360-365. [PDF] This paper showed that Wolbachia infections may alter the arthropod hosts physiology, including increased levels of melanization in adult females and a delayed rate of melanization in the egg stage.
31. De Barro PJ, Murphy B, Jansen CC, Murray J. (2011). The proposed release of the yellow fever mosquito, Aedes aegypti containing a naturally occurring strain of Wolbachia pipientis, a question of regulatory responsibility. Journal of Consumer Protection and Food Safety. DOI 10.1007/s00003-011-0671-x. [PDF] This paper describes the regulatory pathway for assessment and approval of open pilot releases of Wolbachia transinfected Aedes aegypti mosquitoes in Australia.
- 30. Iturbe-Ormaetxe I, Walker T, O’ Neill SL (2011). Wolbachia and the biological control of mosquito-borne disease. EMBO reports (6 May 2011). DOI:10.1038/embor.2011.84 [PDF]
This paper reviews recent progress in the use of Wolbachia to control mosquito-borne diseases.
- 29. McMeniman CJ, Hughes GL, O’Neill SL (2011). A Wolbachia symbiont in Aedes aegypti disrupts mosquito egg development to a greater extent when mosquitoes feed on nonhuman versus human blood. Journal of Medical Entomology 48(1): 76-84. [PDF]
This paper reports that female Ae. aegypti mosquitoes infected with the wMelPop Wolbachia strain had a reduced ability to use non-human blood for egg development. - 28. Moreira LA, Ye YH, Turner K, Eyles DW, McGraw EA, O'Neill SL (2011). The wMelPop strain of Wolbachia interferes with dopamine levels in Aedes aegypti. Parasites & Vectors 2011 4:28. [PDF]
Scientists investigated the possible relationships between dopamine production and the behavioral patterns in mosquitoes associated with Wolbachia infection. - 27. Yeap HL, Mee P, Walker T, Weeks AR, O’Neill SL, Johnson P, Ritchie SA, Richardson KM, Doig C, Endersby NM & Hoffmann AA (2011) Dynamics of the ‘‘Popcorn’’ Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control. Genetics 187: 583–595. [PDF]
This paper describes some of the effects of Popcorn Wolbachia on the survivorship of Aedes aegypti eggs, larvae and adults. - 26. Ritchie SA, Johnson PH, Freeman AJ, Odell RG, Graham N, DeJong, PA, Standfield, GW, Sale, RW & O'Neill Sl (2011) A Secure Semi-Field System for the Study of Aedes aegypti. PLoS Neglected Tropical Diseases 5(3): e988. doi:10.1371/journal.pntd.0000988 [PDF]
This paper describes the secure outdoor cage facility that was constructed in Cairns North Queensland for the testing of Wolbachia infected Aedes aegypti mosquitoes and its important performance characteristics. - 25. Popovici J, Moreira LA, Poinsignon A, Iturbe-Ormaetxe I, McNaughton D & O'Neill SL (2010) Assessing key safety concerns of a Wolbachia-based strategy to control dengue transmission by Aedes mosquitoes. Memórias do Instituto Oswaldo Cruz 105(8) 957-964 [PDF]
This paper describes key safety concerns raised by the general public about the Wolbachia method and how these have been addressed. - 24. Frentiu FD, Robinson J, Young PR, McGraw EA & O'Neill SL (2010) Wolbachia-mediated resistance to dengue virus infection and death at the cellular level. PLoS ONE 5(10) e13398 [PDF]
The paper details the development of a mosquito cell line-based model to dissect the functional and cellular mechanisms of Wolbachia-mediated viral inhibition. We show that cell lines infected with Wolbachia display limited dengue virus replication and protection from virus-induced mortality. The extent of viral inhibition is strongly correlated with Wolbachia density per cell, suggesting competition for cellular resources may underlie this phenotype. - 23. McMeniman CJ & O'Neill SL (2010) A virulent Wolbachia infection decreases the viability of the dengue vector Aedes aegypti during periods of embryonic quiescence. PLoS Neglected Tropical Diseases 4(7) e748 [PDF]
This paper reports on the effects of a life-shortening strain of Wolbachia on key life history traits of Aedes aegypti which provides information on the fitness consequences of infection with this strain of Wolbachia. A key finding is that this Wolbachia strain reduces the time that mosquito eggs can withstand drying and as such might open up a new way to think about how to to use Wolbachia infections for mosquito control in certain ecological contexts. - 22. McNaughton D, Clough A, Johnson P, Ritchie S & O'Neill S (2010) Beyond the 'back yard': Lay knowledge about Aedes aegypti in northern Australia and its implications for policy and practice. Acta Tropica 116(1): 74-80 [Article] [PDF]
- 21. Murphy B, Jansen C, Murray J & De Barro P (2010) Risk Analysis on the Australian release of Aedes aegypti (L.) (Diptera: Culicidae) containing Wolbachia. CSIRO Report [PDF] [Summary]
This report describes a comprehensive risk analysis of releasing Wolbachia infected Aedes aegypti into the Australian environment. The report concludes that there is negligible risk that Aedes aegypti containing Wolbachia would “cause more harm” than the current Australian situation. - 20. Hugo LE, Cook PE, Johnson PH, Rapley LP, Kay BH, Ryan PA, Ritchie SA & O'Neill SL (2010) Field validation of a transcriptional assay for the prediction of age of uncaged Aedes aegypti mosquitoes in Northern Australia. PLoS Neglected Tropical Diseases 4(2): e608 [Article] [PDF]
This paper describes an investigation to determine the robustness of the transcriptional profiling method for predicting the age of Aedes aegypti mosquitoes. The method was successfully applied to mosquitoes that were uncaged and free to engage in natural behaviour. We demonstrate that when applied to populations of mosquitoes, transcriptional age grading could be used to identify a 50% reduction in mosquito lifespan. - 19. Turelli M (2010) Cytoplasmic incompatibility in populations with overlapping generations. Evolution 64(1): 232–241 [Article] [PDF]
Previous mathematical descriptions of the population dynamics of Wolbachia causing cytoplasmic incompatibility assume non-overlapping generations and hence cannot describe the dynamics of strains that shorten life or lengthen development. This paper provides a mathematical framework for determining the initial frequencies needed for life-shortening infections to spread after introduction. - 18. Moreira LA, Iturbe-Ormaetxe I, Jeffery JA, Lu G, Pyke AT, Hedges LM, Rocha BC, Hall-Mendelin S, Day A, Riegler M, Hugo LE, Johnson KN, Kay BH, McGraw EA, van den Hurk AF, Ryan PA & O'Neill SL (2009) A Wolbachia symbiont in Aedes aegypti limits infection with Dengue, Chikungunya, and Plasmodium. Cell 139(7):1268-78 [Article PDF] [supplemental data] [Video Summary]
This paper demonstrates that Aedes aegypti mosquitoes containing Wolbachia have a greatly reduced ability to support infection with dengue virus as well as the unrelated human virus also transmitted by this mosquito, Chikungunya. It also demonstrates a reduction in the ability of the mosquito to be infected with avian malaria parasites. This paper demonstrates an alternative mode of action by which Wolbachia may reduce the ability of the mosquito to transmit dengue in addition to life-shortening. - 17. Osborne SE, Leong YS, O’Neill SL & Johnson KN (2009) Variation in antiviral protection mediated by different Wolbachia strains in Drosophila simulans. PLoS Pathogens 5(11): e1000656 [Article] [PDF]
This paper shows that different Wolbachia strains have varying abilities to protect the fruitfly Drosophila against pathogenic fruitfly viruses. As such not all Wolbachia strains can interfere with viruses. The reason for this is not currently understood. - 16. Moreira LA, Saig E, Turley AP, Ribeiro JMC, O'Neill SL & McGraw EA (2009) Human probing behavior of Aedes aegypti when infected with a life-shortening strain of Wolbachia. PLoS Neglected Tropical Diseases 3(12):e568 [Article] [PDF]
This paper shows that Aedes aegypti infected with life-shortening Wolbachia have a reduced ability to bite people, particularly when they are old. This would reduce their ability to transmit dengue and other pathogens as they are transmitted between people by the bite of the mosquito. This paper also demonstrates that Wolbachia are not present in Aedes aegypti saliva and so cannot be transmitted to people. - 15. Jeffery JAL, Nguyen TY, Vu SN, Le TN, Hoffmann AA, Kay BH & Ryan PA (2009) Characterizing the Aedes aegypti population in a Vietnamese village in preparation for a Wolbachia-based mosquito control strategy to eliminate dengue. PLoS Neglected Tropical Diseases 3(11):e552 [Article] [PDF]
This paper described the distribution and abundance of dengue mosquitoes on Tri Nguyen Island in central Vietnam. Depending on the time of the survey, we found that the average numbers of female mosquitoes could be as high as 43 individuals per house. - 14. Endersby NM, Hoffmann AA, White VL, Lowenstein S, Ritchie S, Johnson PH, Rapley LP, Ryan PA, Nam VS, Yen NT, Kittiyapong P & Weeks AR (2009) Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and exon primed intron crossing markers suggests feasibility of local control options. Journal of Medical Entomology 46(5):1074-83 [Article] [PDF]
This paper investigates the population genetics of the dengue vector, Aedes aegypti, in Australia and Vietnam. It is shown that restricted gene flow occurs between populations of A. aegypti in northern Australia and that samples from Australia are genetically differentiated from Vietnam and Thailand samples. The implications for Wolbachia and other genetic control strategies are discussed. - 13. Evans O, Caragata EP, McMeniman CJ, Woolfit M, Green DC, Williams CR, Franklin CE, O'Neill SL & McGraw EA (2009) Increased locomotor activity and metabolism of Aedes aegypti infected with a life-shortening strain of Wolbachia pipientis. The Journal of Experimental Biology 212(10):1436-41 [Article] [PDF]
Using videography techniques Evans et al. examined the locomotor activity of Wolbachia infected Aedes aegypti relative to uninfected over several adult ages. Wolbachia infection caused slight increases in the activity of the mosquitoes both during day and night but did not disturb circadian rhythms. The infected mosquitoes also show slightly higher metabolic rates. One possible explanation is that the Wolbachia are causing a metabolic drain on the host and the mosquitoes are spending more time seeking sugar water. - 12. Turley AP, Moreira LA, O'Neill SL & McGraw EA (2009) Wolbachia infection reduces blood-feeding success in the dengue fever mosquito, Aedes aegypti. PLoS Neglected Tropical Diseases 3(9): e516 [Article] [PDF]
This paper demonstrates that old mosquitoes infected with life-shortening Wolbachia have difficulty in being able to insert their mouthparts into human skin. They display a “bendy” proboscis trait when they are old – which can be seen here. - 11. Carrington LB, Leslie J, Weeks AR & Hoffmann AA (2009) The popcorn Wolbachia infection of Drosophila melanogaster: can selection alter Wolbachia longevity effects? Evolution 63(10):2648-57 [Article] [PDF]
This paper shows that life shortening effects of Wolbachia in Drosophila can be partly attenuated by the host genetic background following strong selection. - 10. Haygood R & Turelli M (2009) Evolution of incompatibility-inducing microbes in subdivided host populations Evolution 63(2): 432-47 [Article] [PDF]
This paper demonstrates the reliability of simple evolutionary predictions that ignored host-population subdivision. Even extreme subdivision does not alter the prediction that natural selection on Wolbachia acts primarily to increase the number of infected progeny produced by infected mothers. - 9. McMeniman CJ, Lane RV, Cass BN, Fong AWC, Sidhu M, Wang YF & O'Neill SL (2009) Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti. Science 323(5910): 141-144 [Article] [PDF]
(Commentary: Perspectives (2009) Science 323(5910): 51-52)[Article] [PDF]
(Commentary: Research Highlights (2009) Nature Biotechnology 27(2): 152)[PDF] This paper demonstrates the successful transfer of life-shortening Wolbachia from the fruitfly into Aedes aegypti. In the mosquito it roughly halves adult female lifespan. - 8. Williams CR, Johnson PH, Long SA, Rapley LP & Ritchie SA (2008) Rapid estimation of Aedes aegypti population size using simulation modeling, with a novel approach to calibrtion and field validation. Journal of Medical Entomology 45(6): 1173-79 [Article] [PDF]
The field validation of model that predicts Aedes aegypti population dynamics is described here. By using readily available breeding site and meteorological data, dengue vector population size and structure can be predicted. This validated model (known as CIMSiM) is vital for developing and testing strategies for Wolbachia field release. - 7. McMeniman CJ, Lane AM, Fong AW, Voronin DA, Iturbe-Ormaetxe I, Yamada R, McGraw EA & O'Neill SL (2008) Host adaptation of Wolbachia after long-term serial passage in mosquito cell lines. Applied and Environmental Microbiology 74(22):6963–69 [Article] [PDF]
This paper describes the process of maintaining the life-shortening Wolbachia in mosquito cell culture to help adapt it to the mosquito intracellular environment prior to transfer into Aedes aegypti. - 6. Hedges LM, Brownlie JC, O'Neill SL, & Johnson KN (2008) Wolbachia and virus protection in insects. Science 322(5902):702 [Article] [PDF]
(Commentary: Research Highlights (2008) Nature 456: 4) [PDF] (Commentary: Perspectives (2008) Science 322: 1199-1200) [PDF] This paper demonstrates that Wolbachia can prevent pathogenic viruses from killing the fruitfly Drosophila – by interacting with the ability of the viruses to grow in the insect when Wolbachia is present. - 5. Jansen VA, Turelli M & Godfray HC (2008) Stochastic spread of Wolbachia. Proceedings of the Royal Society B 275(1652):2769-76 [Article] [PDF]
Mathematical descriptions of the population dynamics of Wolbachia generally ignore the stochastic effects associated with finite population size. This paper shows that if many infected individuals are released such effects are indeed negligible for population sizes on the order of hundreds. - 4. Iturbe-Ormaetxe I & O’Neill SL (2007) Wolbachia-host interactions: linking phenotype to genotype. Current Opinion in Microbiology 10(3):221-4 [Article] [PDF]
A general review article on Wolbachia insect interactions. - 3. Cook PE, Hugo LE, Iturbe-Ormaetxe I, Williams CR, Chenoweth SF, Ritchie SA, Ryan PA, Kay BH, Blows MW & O'Neill SL (2007) Predicting the age of mosquitoes using transcriptional profiles. Nature Protocols 2:2796-2806 [Article] [PDF]
This paper describes in detail the protocol for applying the transcriptional age-grading method to Aedes aegypti. - 2. Cook PE, McMeniman CJ & O’Neill SL (2007) Modifying Insect Population Age Structure to Control Vector-Borne Disease. In: Aksoy S, ed. Transgenesis and the Management of Vector-Borne Disease. Austin: Landes Bioscience [PDF]
This paper provides a review of the approach of reducing mosquito lifespan to reduce pathogen transmission.
- 1. Cook PE, Hugo LE, Iturbe-Ormaetxe I, Williams CR, Chenoweth SF, Ritchie SA, Ryan PA, Kay BH, Blows MW & O'Neill SL (2006) The use of transcriptional profiles to predict adult mosquito age under field conditions. Proceedings of the National Academy of Sciences USA 103(48):18060-18065 [Article] [PDF] (Commentary: Research Highlights (2006) Nature 444, 7118: 404-5) [PDF]
This paper describes a new method to predict the age of field caught mosquitoes. It uses a transcriptional profiling approach to predict mosquito age and is considered an important tool for measuring the potential effect of Wolbachia on mosquito lifespan in the field. |
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